RPS6KB1 and Insulin resistance: The metabolic implications of elevated BCAAs in insulin resistance (IR) are unclear, but overactivation of ribosomal protein S6 kinase B1 (S6K1)-related signaling pathway may contribute to IR.<h4>Objective</h4>To investigate the impact of 2 dietary protein interventions on IR and molecular signaling in skeletal muscle (acutely) and adipose tissue (18-week period) in overweight/obese individuals.<h4>Methods</h4>The acute study included ingestion of 50 g protein (MPD), 100 g protein (HPD), or 50 g of protein with added fat (MPDAF) on 3 different occasions with muscle biopsies before and after.