Furthermore, the 493 TF-6mRNA-183miRNA regulatory network of DN and 111 TF-4mRNA-33miRNA regulatory network of DR were constructed, among them, hsa-mir-101-3p was the common miRNA for ZNF223, NMNAT2 and TTC25, and EGR1 was the key TF which could regulate ATP4A, GAS6, METTL27, NMNAT2 and TTC25 at the same time.<h4>Conclusion</h4>This study revealed the potential molecular mechanisms of diagnostic genes in DN and DR, which could provide novel insights for the clinical diagnosis and treatment of DN and DR. This evidence concerns the gene ATP4A and liver dysplastic nodule.