EGFR and central nervous system cancer: Subsequent BLI assays validated a direct physical interaction between zeylenol and EGFR, and biological validation demonstrated that zeylenol treatment significantly suppressed GBM cell proliferation and migration while promoting apoptosis, supporting its therapeutic potential as a novel EGFR inhibitor.<h4>Discussion</h4>This study identifies EGFR as a causally relevant therapeutic target in glioma and highlights tremulacin and zeylenol as promising natural EGFR inhibitors, warranting further preclinical development of these compounds as novel glioma therapeutics.