In this context, the effects of a high-fat diet (HFD) induced obesity on sodium-dependent glucose co-transporter 1 (SGLT1), Na<sup>+</sup>/H<sup>+</sup> exchanger 3 (NHE3), and Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup> exchangers (DRA/PAT1), the primary glucose, sodium, and chloride absorptive pathways in mice small intestinal villus cells, were investigated. This evidence concerns the gene SLC26A3 and obesity due to melanocortin 4 receptor deficiency.