In this context, we propose repositioning EuroFlow panels within a new set of analyses to assess leukemia stem-like phenotypes for AML prognostic monitoring.<h4>Methods</h4>We performed a retrospective study with AML patients at the Hospital de Clínicas de Porto Alegre between 2015 and 2023.<h4>Results</h4>Our findings highlight the relevance of leukemia stem cell-like phenotypes, particularly the CD123+, CD34+CD123+, and CD34+CD38- subpopulations, for complementary prognostication of adult and elderly patients. This evidence concerns the gene CD38 and acute myeloid leukemia.