Western blot validation in prostate and ovarian cancer cell lines confirmed our computational analysis.<h4>Results</h4>POFUT1 showed significant overexpression in 16 of 33 cancer types (FDR-adjusted <i>p</i> < 0.05), with the highest elevation in BRCA (<i>breast invasive carcinoma</i>; log2FC = 2.31) and LUAD (<i>lung adenocarcinoma</i>; log2FC = 2.1). Here, POFUT1 is linked to lung adenocarcinoma.