TTK and breast cancer: To dissect the transcriptional regulation of the key hub gene TTK, we first predicted potential upstream transcription factors using the JASPAR database; binding specificity was then validated through in silico motif analysis, luciferase reporter assays, and chromatin immunoprecipitation followed by quantitative PCR (ChIP-qPCR).<h4>Results</h4>The mitotic kinase TTK is significantly overexpressed in TNBC compared with non-TNBC breast cancers.