Despite showing anti-tumorigenic activity, the role of ligustilide (LIG) in BC treatment remains limited.<h4>Aim</h4>To evaluate LIG-mediated autophagy inhibition in DOX-treated MCF-7, MCF-7/DOX, and MDA-MB-231 cells, and investigate LncRNA H19's role in BC resistance.<h4>Methods</h4>The three cell lines were treated with DOX, LIG, or both of them. Here, H19 is linked to breast cancer.