In macrophages, rutin showed anti-inflammatory and anti-glycolytic effects, and co-treatment with STF-31 showed no additive effects, suggesting a GLUT1-dependent mechanism.<h4>Conclusion</h4>KHF exerts anti-inflammatory and anti-fibrotic effects in AE-IPF, partly by modulating GLUT1-mediated glycolysis and regulating the GLUT1/HIF-1α axis, with rutin as a key bioactive component. Here, HIF1A is linked to idiopathic pulmonary fibrosis.