In addition, TEN significantly increased the expression of desuccinylase SIRT5, leading to reduced global succinylation and specific histone H3 lysine 122 succinylation (H3K122su) while simultaneously modulating the ERK/JNK signaling pathway in HCC cells.<h4>Conclusion</h4>TEN exerts its anti-tumor effect in HCC cells by targeting SIRT5-succinylation. Here, MAPK8 is linked to hepatocellular carcinoma.