RT-qPCR was used to quantify IL-6 and TNF-α mRNA expression in joint and lung tissues, while Western blot analysis was used to detect TGF-β1 and α-SMA protein expression in lung tissue.<h4>Results</h4>TWG ameliorated both arthritis severity and pulmonary injury in a dose-dependent manner, with the strongest effects observed in the TWG-H group, showing efficacy comparable to Pred group. The gene discussed is ACTA1; the disease is Arthritis.