CD28 and prediabetes syndrome: <b>Results:</b> In a case study using anti-CD3/anti-CD28-stimulated peripheral blood mononuclear cell cytokine profiles from subjects with prediabetes at 20 and 72 h (33 samples per time point), CytokineProfile supported same-dataset comparison with MetaboAnalyst and CytokineExplore, generated consistent group separation in supervised analyses, and provided complementary volcano, dual-flashlight, and machine-learning-based feature results.