The technique chosen for the immunohistochemical study of the samples was microdensitometry.<h4>Results</h4>By analyzing tissue samples, it investigates the presence of Transforming Growth Factor Beta (TGF-β), Vascular Endothelial Growth Factor (VEGF), Tumor Necrosis Factor Alpha (TNF-α) in angiogenesis, fibrosis, and inflammation-three critical processes in the development of malignancies. This evidence concerns the gene TNF and cancer.