mB7-H4 expression on circulating immune cell subsets was determined by flow cytometry, while plasma sB7-H4 concentration was quantified using enzyme-linked immunosorbent assay (ELISA).<h4>Results</h4>Compared to HC, patients with relapsing MG exhibited significantly increased mB7-H4 expression on CD4<sup>+</sup> T cells and CD14<sup>+</sup> monocytes (p < 0.05). This evidence concerns the gene CD4 and myasthenia gravis.