In contrast, ITPKA knockdown significantly promoted the proliferation, migration, and invasion capabilities of GBM cells in the two GBM cell lines <i>in vitro</i> and the progression of subcutaneous xenograft tumors in these two GBM cell lines in nude mice.<h4>Conclusions</h4>Low expression of ITPKA in glioma tissues correlates with GBM progression, indicating that it may act as a tumor suppressor gene and is a candidate biomarker for the molecular diagnostic and prognostic evaluations of glioblastoma. The gene discussed is ITPKA; the disease is glioma.