Proteomic and immunofluorescence analyses showed that PA downregulated Wnt pathway proteins (Dvl3, Tcf7l2) and mesenchymal EMT markers (N-cadherin, MMP9) while upregulating the Wnt inhibitor APC and epithelial marker E-cadherin, collectively reversing HP-induced Wnt/EMT hyperactivation.<h4>Conclusion</h4>PA activates PXR through both direct and indirect mechanisms, thereby suppressing Wnt/β-catenin signaling and EMT, and ultimately inhibiting the initiation and progression of H. pylori-associated gastric cancer. Here, NR1I2 is linked to gastric cancer.