Concurrently, Fatty acid content in cardiac tissues was evaluated from all three groups using targeted metabolomics, with an assessement of the NF-κB/NLRP3/pyroptosis signaling pathway in each group via western blot and immunohistochemistry.<h4>Results</h4>The bioinformatics analysis was conducted using GSE97320 dataset found the activation of the NF-κB/NLRP3/pyroptosis signaling pathway in MI patients. This evidence concerns the gene NFKB1 and myocardial infarction.