Mechanistically, tRF-21-Z4NY2V7KE directly suppressed MYBL1 expression, as confirmed by luciferase assays.<h4>Conclusions</h4>Our study provides the first comprehensive tsRNA-mRNA regulatory network in asthma, uncovering novel post-transcriptional mechanisms in bronchial epithelial cells. This evidence concerns the gene MYBL1 and asthma.