Finally, the immunogenic cell death (ICD) and reprogrammed immune cells of Alb-TAC-treated tumors were carefully characterized.<h4>Results</h4>Alb-TAC#2 containing the AE-Mal linker exhibited rapid albumin binding, accelerated esterase-responsive activation, and enhanced tumor accumulation compared to ARV-771 and Alb-TAC#1 due to its flexible chemical structure. The gene discussed is ALB; the disease is neoplasm.