This study is aimed at clarifying the genetic effect of glycolytic pathway-related genes on the occurrence and development of LC.<h4>Methods</h4>In this two-stage case-control study, we enrolled 300 LC patients and 600 healthy controls, as well as 1248 case-control pairs from several hospitals in Guangzhou, to evaluate the association between the genetic variations of glycolysis-related genes (<i>GLUT1</i> rs1385129G>A, <i>GLUT11</i> rs6003939A>C, <i>GLUT12</i> rs1484180G>A and <i>ENO2</i> rs11064467C>T) and the risk of LC. This evidence concerns the gene SLC2A12 and laryngotracheoesophageal cleft.