Moreover, SIRT1 binds to ASC in the cytoplasm to inhibit its acetylation, thereby inhibiting the binding of ASC to NLRP3.<h4>Conclusion</h4>In conclusion, Propofol promotes SIRT1-mediated deacetylation to inhibit RTEC pyroptosis via FOXO3a/ASC/NLRP3 axis and alleviate AOLT-induced kidney injury. The gene discussed is FOXO3; the disease is kidney injury.