CD8A and cancer: TRACE scores were observed to be significantly higher in exhausted CD8<sup>+</sup> T cells in tumors but not in exhausted cells in normal adjacent or non-cancer samples, suggesting specificity towards identifying tumor-antigen experienced T cells.<h4>Conclusion</h4>TRACE is a tumor reactivity scoring algorithm released with open model weights that can be applied to tissue or blood single-cell RNAseq datasets.