A total of 54,230 cells across all experimental groups were analyzed using integration and Uniform Manifold Approximation and Projection (UMAP) clustering to identify transcriptionally distinct cell populations and HIV-associated changes.<h4>Results</h4>HIV infection profoundly altered lung cellular composition marked by expansion of CD4<sup>+</sup>, CD8<sup>+</sup> T-cells, B cells, and non-classical monocytes, alongside reduced fibroblast abundance. Here, CD4 is linked to HIV infectious disease.