Alterations of genes that encode for connexins can lead to changes in cell ion content and cause hearing impairment.<h4>Methods</h4><i>GJB2</i> gene polymorphisms (c.71G>A, p.Trp24*rs104894396; c.457G>A, p.Val153Ile, rs111033186; c.380G>A, p.Arg127His, rs111033196; c.109G>A, p.Val37Ile, rs72474224; and c.269T>C, p.Leu90Pro, rs80338945) were analyzed in the Roma population of Croatia. This evidence concerns the gene GJB2 and hearing loss disorder.