<b>Methods:</b> In this study, we generated induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient carrying a novel p.N989I (c.2966A>T) variant of <i>MYPN</i> and used iPSC-derived cardiomyocytes to examine the impact of the variant on biophysical characteristics and transcriptomic profile. This evidence concerns the gene MYPN and hypertrophic cardiomyopathy.