ATG5 and hearing loss disorder: In vitro, deficiency of either <i>Atg5</i> or <i>Foxa3</i> in primary KO cells resulted in comparable reductions in LAMP1-positive puncta.<h4>Conclusions</h4>These findings support a model wherein the ATG5-FOXA3 axis contributes to lysosomal biogenesis in developing KO cells, with implications for understanding mechanisms of congenital sensorineural hearing loss.