Furthermore, AS was proven to exert a hepatoprotective effect by inhibiting inflammation and ferroptosis, along with weakening the advanced glycation end product-receptor for advanced glycation end products (AGE-RAGE) pathway and the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway <i>in vivo</i> and <i>in vitro</i>.<h4>Conclusion</h4>This study first elucidates the mechanism through which AS ameliorates MASH through integrated multi-omics analysis, providing experimental evidence for further development of natural therapeutic agents. The gene discussed is RENBP; the disease is metabolic dysfunction-associated steatohepatitis.