<h4>Background</h4>Fibrosis and inflammation frequently emerge soon after ileal resection for strictures in Crohn's disease (CD).<h4>Objective</h4>This study examined the profibrotic and proinflammatory effects of conditioned media from TNF and IFN-γ (T+I)-exposed healthy and inactive CD ileal enteroids (CDiE) on subepithelial myofibroblasts (SEMF), modeling early post-ileal resection conditions in CD.<h4>Methods</h4>Ileal enteroids from healthy subjects (HE) and inactive CD patients (CDiE) were exposed to T+I for 6 hours, followed by an 18-hour conditioning period. This evidence concerns the gene IFNG and Cowden disease.