Conditioned media from T+I-exposed enteroids altered HIMF-HC (healthy control) and HIMF-CD mRNA expression, upregulating proinflammatory genes (<i>IL1B</i>, <i>IL6</i>, <i>MCP1</i>, and <i>IKBA</i>) while downregulating profibrotic genes (<i>COL1A1</i>, <i>ACTA2</i>, <i>TGFB</i>, and <i>SRF</i>).<h4>Conclusion</h4>Cytokine-exposed HE- and CDiE-derived conditioned media promoted inflammation while suppressing fibrosis-associated gene expression in subepithelial myofibroblasts. Here, IL6 is linked to hereditary elliptocytosis.