IL-13, TNF-α, IFN-γ, and MCP-1 levels positively associated with the EASI, SCORAD, and IGA (<i>r</i> > 0.5; <i>P</i> < .05) in both allergen-challenged and lesional skin.<h4>Conclusions</h4>Prednisolone modulated a broad range of inflammatory pathways in acute versus chronically inflamed AD skin. Here, TNF is linked to Alzheimer disease.