Cell-cell communication and pseudotime analyses further suggested that tnf-tnfrsf1b signaling and progressive upregulation of mapk6/mapk11 along myeloid lineages contribute to the early splenic antibacterial response.<h4>Conclusion</h4>This study provides a genome to single-cell view of MAPK family organization and immune function in bighead carp, and identifies mapk6 and mapk11 as potential mediators of the early spleen response to bacterial infection. Here, MAPK11 is linked to bacterial infectious disease.