Specifically, OCP-3 reduced fasting blood glucose from 31.3 to 17.2 mM and restored insulin levels to 10.34 mU/L, and improved oral glucose tolerance (AUC = 39.83 h·mmol/L), while maintaining an excellent safety profile in toxicological assessment.<h4>Discussion</h4>These findings not only validate the traditional use of <i>Orobanche cumana</i> but also establish OCP-3 as a safe and effective candidate for diabetes treatment, offering a sustainable approach to valorizing this agricultural plant. Here, INS is linked to diabetes mellitus.