We investigated whether the induction of NAFLD can cause dysregulation in the hepatic IL-1β/iNOS and IL-1β/CD45 axes of inflammation and fibrosis, as well as in endogenous metabolites (lipids, glucose, and insulin) and apoptosis, in the presence and absence of the flavonoid quercetin.<h4>Methods</h4>The model group of rats was fed with a high-fat and high-carbohydrate diet (HFCD) for 4 weeks. The gene discussed is NOS2; the disease is metabolic dysfunction-associated steatotic liver disease.