NPM1 and acute myeloid leukemia: In this study, we present a simple, rapid, step-by-step multiplex PCR protocol combined with capillary electrophoresis for the detection of two of the most prevalent molecular alterations in AML: nucleophosmin 1 (<i>NPM1</i>) mutations and Fms-like tyrosine kinase 3 internal tandem duplications (<i>FLT3</i>/ITD).