In this study, we present a simple, rapid, step-by-step multiplex PCR protocol combined with capillary electrophoresis for the detection of two of the most prevalent molecular alterations in AML: nucleophosmin 1 (<i>NPM1</i>) mutations and Fms-like tyrosine kinase 3 internal tandem duplications (<i>FLT3</i>/ITD). The gene discussed is FLT3; the disease is acute myeloid leukemia.