RT-qPCR validation in individual samples confirmed statistically significant differences for <i>ANKRD22</i> (upregulated) and <i>E2F7</i> (downregulated) in infertile patients, while other angiogenesis-related genes, including <i>ANGPT1</i>, <i>ANGPT2</i> and <i>THBS1</i>, showed consistent but non-significant expression trends, suggesting alterations in angiogenesis-related processes within the follicular microenvironment. The gene discussed is ANGPT2; the disease is Infertility.