In contrast, diffuse PD-L1 expression was associated with abundant stromal and intraepithelial infiltration of CD8<sup>+</sup> T cells and FOXP3<sup>+</sup> Tregs.<h4>Conclusions</h4>High-grade tumor budding is associated with LNM in stage IB1 CSCC. The gene discussed is CD8A; the disease is neoplasm.