F8 and hemophilia A: Mini-circle DNA constructs (with the bacterial backbone excised) encoding either wild-type FVIII or E1984V-FVIII were delivered <i>via</i> hydrodynamic tail vein injection into FVIII-KO mice, a typical murine model of hemophilia A. Coagulation outcomes (aPTT, FVIII activity, tail-clip assay) and transgene persistence were monitored over 26 weeks.