Antioxidant activity in the intestine, measured by SOD and GSH-Px, was lower in Group III compared to Group I. Conversely, Group IV showed significant improvements in biochemical, histopathological, and immunohistochemical outcomes, alongside reduced intestinal damage caused by E. coli.<h4>Conclusion</h4>This study demonstrates that E. coli infection in mice increases IL-6 and MMP-9 levels while decreasing intestinal antioxidants. The gene discussed is MMP9; the disease is escherichia coli infection.