AKT1 and gestational diabetes: Mechanistic investigations revealed that NTF4 contributes to cellular injury by activating the PI3K/AKT signaling pathway, while the PI3K inhibitor LY294002 further amplified the protective effects of NTF4 silencing.<h4>Conclusions</h4>This study identified the role of NTF4 in promoting trophoblast injury by activating the PI3K/AKT signaling pathway in high-glucose-stimulated HTR-8/SVneo cells, which provides a preliminary experimental basis for exploring its potential role in the pathogenesis of GDM.