<h4>Objective</h4>This study aims to investigate the role of neurotrophin-4 (NTF4) in gestational diabetes mellitus (GDM) and to elucidate the underlying mechanism by which NTF4 regulates high glucose-induced apoptosis and inflammation in HTR-8/SVneo cells via the PI3K/AKT signaling pathway.<h4>Methods</h4>Transcriptomic analysis combined with public database screening revealed that NTF4 is significantly upregulated in placental tissues from GDM patients. The gene discussed is NTF4; the disease is gestational diabetes.