BMI1 and Infertility: BMI1 levels, measured by immunohistochemistry (IHC), quantitative real-time PCR (qPCR), and western blot (WB), demonstrated diagnostic value by Receiver Operating Characteristic (ROC) analysis and predicted superior reproductive outcomes in a two-year infertile patient cohort (<i>n</i> = 50).<h4>Results</h4>BMI1 expression was significantly downregulated at both the mRNA and protein levels in the SA compared to the EA group (<i>p</i> < 0.05), with predominant localization in the endometrial epithelium.