Following testing, hippocampal tissues were collected for molecular analyses, including real-time PCR for TLR4, MyD88, and NF-κB expression, and ELISA quantification of IL-1β and IL-6 levels.<h4>Results</h4>Reserpine induced robust anxiety-like behaviours, accompanied by significant upregulation of TLR4, MyD88, and NF-κB expression and increased hippocampal IL-1β and IL-6. This evidence concerns the gene MYD88 and Anxiety.