Western blotting was used to analyse the effects of BAI and IGF-1 co-treatment on the phosphorylation level of the pathway and its downstream cycling, apoptosis and migration related proteins.<h4>Results</h4>Integrated analyses implicated the inhibitory effect of BAI on choroidal melanoma may be related to the cell cycle, apoptosis and critical signaling pathways, particularly the PI3K/AKT/mTOR axis. Here, AKT1 is linked to choroidal melanoma.