Experimental analyses indicated higher mRNA levels of HES4, SHROOM2, and OXCT1 in CRC tissues than in adjacent normal tissues (qRT-PCR, n = 10 pairs), and increased protein expression of HES4 and SHROOM2 (western blotting, n = 6 pairs).<h4>Conclusions</h4>This study identified hypoxia- and pyroptosis-related molecular patterns and a six-gene prognostic signature in CRC. This evidence concerns the gene HES4 and colorectal carcinoma.