Enhancement with rabies virus glycoprotein (RVG29) has shown improved brain-related efficacy.<h4>Methods</h4>In this study, we developed CD70R CAR-T cells by modifying anti-CD70 CAR-T cells with RVG29 and tested their tumor-killing ability <i>in vitro</i>.<h4>Results</h4>Transcriptomic profiling via RNA-seq revealed the activated signaling pathways in modified CD70R CAR-T cells. This evidence concerns the gene CD70 and neoplasm.