However, data on real-world utilization patterns of toxoplasmosis diagnostic tests remain limited, particularly in settings where the disease is relatively uncommon.<h4>Methods</h4>We retrospectively analyzed laboratory data for Toxoplasma gondii PCR, IgG, and IgM assays performed for clinical purposes between January 2020 and December 2024 at a large referral laboratory in Korea. This evidence concerns the gene CD40LG and toxoplasmosis.