Validation <i>via</i> SMR and qRT-PCR assays demonstrated significant dysregulation of four target genes (<i>CXCR1</i>, <i>HECW2</i>, <i>ATP13A4</i>) (<i>P</i> < 0.05).<h4>Discussion</h4>This study suggests that <i>CXCR1</i>, <i>HECW2</i>, and <i>ATP13A4</i> may be involved in CRC development, providing a reference for targeted and immunotherapy research. This evidence concerns the gene CXCR1 and colorectal carcinoma.