However, its effect on asthma inflammation remains unclear.<h4>Methods</h4>Our study employed an integrative strategy-combining network pharmacology, molecular docking, molecular dynamics simulations, and <i>in vivo</i> experiments-to investigate 3PO's anti-inflammatory action in asthma.<h4>Results</h4>Firstly, the construction of a protein-protein interaction (PPI) network identified nine hub genes: TNF, IL6, BCL2, IL1B, CASP3, RELA, MAPT, MAOB, and MAOA. Here, TNF is linked to asthma.