Here, we questioned whether TGF-β-regulated apoptotic/autophagic phenotypes are recapitulated in a preclinical in vitro 3D spheroid culture model of human U87 GBM-derived cells, and how metabolic alterations affect such phenotypes.<h4>Methods</h4>3D U87 spheroids were cultured using the hanging drop method. Here, TGFB1 is linked to glioblastoma.