<h4>Background</h4>TONSL is involved in various biological processes such as maintaining genomic stability and promoting tumor progression.<h4>Aims</h4>The purpose of this article is to comprehensively clarify the expression of TONSL in Pan-Cancer, explore the association between TONSL expression and tumor tissues, prognosis, and immune infiltration in the tumor microenvironment, and clarify the interaction and combined effect between TONSL and MMS22L. Here, TONSL is linked to neoplasm.