Gab2, a tyrosine kinase substrate, is implicated in glioma pathogenesis; however, the mechanism by which GS-Rh2 might inhibit glioma cell migration and invasion via the Gab2/Akt2 pathway remains unexplored.<h4>Objective</h4>To investigate the effects and mechanisms of GS-Rh2 on glioma cell migration and invasion through the Gab2/Akt2 signaling pathway.<h4>Methods</h4>U251 glioma cells were treated with GS-Rh2 <i>in vitro</i>. This evidence concerns the gene RHAG and central nervous system cancer.