HE staining assessed brain invasion, and western blot detected Gab2 and Akt2 expression in tumor tissues.<h4>Results</h4>GS-Rh2 significantly inhibited U251 cell proliferation, migration, and invasion both <i>in vitro</i> and <i>in vivo</i> (<i>P</i> < 0.05), while no significant effects were observed in siGab2/U251 cells. The gene discussed is GAB2; the disease is neoplasm.