In this study, we examined the direct antitumor effects and the impact on the tumor microenvironment of FH535, a small-molecule inhibitor of Wnt/β-catenin and PPARδ signaling, in human CRC cells.<h4>Methods</h4>We treated HCT116 and HT29 colorectal cancer cell lines with different doses of FH535. The gene discussed is PPARD; the disease is neoplasm.